1x thermopol reaction buffer (New England Biolabs)

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New England Biolabs 1x thermopol reaction buffer
1x Thermopol Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 2335 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+thermopol+reaction+buffer/ThermoPol+Reaction+Buffer/pmc12993374-147-70-74
Average 98 stars, based on 2335 article reviews

1x thermopol reaction buffer - by Bioz Stars, 2026-10

98/100 stars

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Modification:

Article Title: Multifaceted mRNA analysis using programmed RNA cleavage by Mucilaginibacter paludis Argonaute
Article Snippet: .. 200 ng of Fluc RNA (∼1.8 kb, ∼0.37 pmol), a shortened version of the pTsin saRNA (pSG90; ∼1.7 kb, ∼0.37 pmol), or Epo RNA with a modified sequence (pSG95; ∼0.7 kb, ∼0.86 pmol), all synthesized using in vitro transcription (IVT) (New England Biolabs, #E2040) according to the manufacturer’s instructions, were cleaved with MpaAgo in a 10 μL reaction at 50°C for either 15 minutes (single and double cleavages, 1:5 substrate: Ago molar ratio) or 30 minutes (multiplexing, 1:10 substrate: Ago ratio) in 1x ThermoPol® reaction buffer (New England Biolabs #B9004S). ..

Sequencing:

Synthesized:

In Vitro:

Multiplexing:

Incubation:

Article Title: Multifaceted mRNA analysis using programmed RNA cleavage by Mucilaginibacter paludis Argonaute
Article Snippet: The capping reactions were subsequently column purified (New England Biolabs #T2040S). .. 200 μl reactions containing 3.5 pmol of capped and uncapped Fluc or pSG90 mRNA were then incubated for 15 min at 50°C in 1x ThermoPol® reaction buffer, 30% glycerol, 160 units of Murine RNase Inhibitor (New England Biolabs #M0314S), 67.4 pmol MpaAgo, and 66.4 pmol guide (A1: /5Phos/ATCTCCTTCTTAAAGT, A1.2: /5Phos/ TCCTTCTTAAAGTTAAAC, D891: /5Phos/ TATATCTCCTTCTTAA targeting Fluc mRNA, or D831: /5Phos/GTCGGCTGTTTGATTC, D892: /5Phos/ TTGATTCAATAGTGTG targeting pSG90 mRNA). ..

Polymerase Chain Reaction:

Article Title: Genetic diversity and morphological variation of aibika [Abelmoschus manihot (L.) Medik.] in Melanesia
Article Snippet: Before the PCR reaction, we also had to purify a few recalcitrant samples with AMPure XP magnetic beads (Beckman Coulter, A63881) with a 0.8X bead/DNA ratio. .. PCR was performed separately for each primer pair in 10 μL final solution, containing 5–10 ng template DNA, 0.5 mM MgCl2, 200 μM dNTPs, 0.2 μM reverse primer, 0.16 μM M13-tailed forward primer, 0.2 μM M13 primer fluorescently labelled with FAM, VIC, PET, or NED (Applied Biosystems, Foster City, California, USA), 0.04 ng bovine serum albumin, 0.75X Q-Product (Qiagen, Hilden, Germany) and 0.06 U Taq DNA polymerase with 1X ThermoPol® reaction buffer (New England Biolabs, Ipswich, MA, USA). .. PCR was performed on a thermocycler Mastercycler® nexus, Eppendorf AG (Hamburg, Germany) with the following settings: initial denaturation at 95 °C for 5 min; 10 cycles of touchdown, starting at 95 °C for 30 s, then annealing temperature (Ta) plus 5 °C (0.5 °C decrease at each cycle) for 1 min, and 68 °C for 1 min; 23 cycles at 95 °C for 30 s, Ta for 1 min, and 68 °C for 1 min; and a final elongation step at 68 °C for 10 min. Allele size was determined after separation on an ABI 3500 xL Genetic Analyzer (Applied Biosystems, Foster City, California, USA).

Article Title: Shotgun sequencing data and SSR mining data of aibika ( Abelmoschus manihot , Malvaceae)
Article Snippet: We also had to purify a few recalcitrant samples with AMPure XP magnetic beads (Beckman Coulter, A63881) with a 0.8X bead/DNA ratio. .. PCR was performed separately for each primer pair in 10 μL final solution, containing 5–10 ng template DNA, 0.5 mM MgCl 2 , 200 μM dNTPs, 0.2 μmol reverse primer, 0.16 μmol M13-tailed forward primer, 0.2 μmol M13 primer fluorescently labelled with FAM, VIC, PET, or NED (Applied Biosystems, Foster City, California, USA), 0.04 ng bovine serum albumin, 0.75X Q-Product (Qiagen, Hilden, Germany) and 0.06 U Taq DNA polymerase with 1X ThermoPol® reaction buffer (New England Biolabs, Ipswich, MA, USA). .. PCR was performed on a thermocycler Mastercycler® nexus, Eppendorf AG (Hamburg, Germany) with the following settings: initial denaturation at 95°C for 5 min; 10 cycles of touchdown, starting at 95°C for 30 s, then annealing temperature (T a ) plus 5°C (0.5°C decrease at each cycle) for 1 min, and 68°C for 1 min; 23 cycles at 95°C for 30 s, T a for 1 min, and 68°C for 1 min; and a final elongation step at 68°C for 10 min. Allele size was determined after separation on an ABI 3500 xL Genetic Analyzer (Applied Biosystems, Foster City, California, USA).

Article Title: Morphological variation and genetic diversity of breadfruit [Artocarpus altilis (Parkinson) Fosberg] in Vanuatu
Article Snippet: Breadfruit, Artocarpus altilis (Parkinson) Fosberg, is a versatile tree crop widely distributed in Oceania and tropical regions.. It produces starchy fruits that contribute to food security in Pacific Island countries.. In Vanuatu, where it is considered as a secondary food crop, a better understanding of the diversity of this species is a prerequisite for setting up a genetic improvement strategy.

Article Title: Shotgun sequencing data and SSR mining data of aibika ( Abelmoschus manihot , Malvaceae)
Article Snippet: Before the PCR reaction, we also had to purify a few recalcitrant samples with AMPure XP magnetic beads (Beckman Coulter, A63881) with a 0.8X bead/DNA ratio. .. PCR was performed separately for each primer pair in 10 μL final solution, containing 5-10 ng template DNA, 0.5 mM MgCl 2 , 200 μM dNTPs, 0.2 μmol reverse primer, 0.16 μmol M13-tailed forward primer, 0.2 μmol M13 primer fluorescently labelled with FAM, VIC, PET, or NED (Applied Biosystems, Foster City, California, USA), 0.04 ng bovine serum albumin, 0.75X Q-Product (Qiagen, Hilden, Germany) and 0.06 U Taq DNA polymerase with 1X ThermoPol® reaction buffer (New England Biolabs, Ipswich, MA, USA). .. PCR was performed on a thermocycler Mastercycler® nexus, Eppendorf AG (Hamburg, Germany) with the following settings: initial denaturation at 95 °C for 5 min; 10 cycles of touchdown, starting at 95 °C for 30 s, then annealing temperature (T a ) plus 5 °C (0.5 °C decrease at each cycle) for 1 min, and 68 °C for 1 min; 23 cycles at 95 °C for 30 s, T a for 1 min, and 68 °C for 1 min; and a final elongation step at 68 °C for 10 min. Allele size was determined after separation on an ABI 3500 xL Genetic Analyzer (Applied Biosystems, Foster City, California, USA).

Positron Emission Tomography:

Purification:

Article Title: Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR
Article Snippet: DNA concentration was determined by using the 4150 TapeStation System (Agilent) as described above. .. In the next step, DNA was repaired by using 0.35 nM purified DNA, 0.1 μL PreCR Repair mix (NEB) with 100 μM of dNTP (each), 1x NAD + (provided by the manufacturer) and 1x ThermoPol reaction buffer (NEB) to reach 20 μL reaction volume. ..

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